Introduction
The ViraTrapTM lentivirus maxi purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Viral particles can be purified from cell culture of 4 to 5 T75 flasks per column.
The viruses are first applied to a purification column and then further purified and concentrated through a concentration unit
Each column can be regenerated for purifying the same lentivirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
The Guaranteed shelf life is 12 months from the date of purchase. Store LV column at 4°C
Before Starting
Familiar with each step by reading this menu and prepare all materials for the procedure.
Safety Considerations
The lentiviral infected cell culture and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.
Materials Required but Not Supplied
- Standard TC centrifuge
- Swing bucket rotor.
- 0.45 μm filter unit
- Rack holder for column
Kit Contents
| Catalog# |
V1270-01 |
V1270-02 |
| Preps |
4 |
10 |
| LV Columns |
2 |
5 |
| Press-On Cap |
4 |
10 |
| Centrifugal Device |
4 |
10 |
| 50 mL Conical Tube |
8 |
20 |
| Buffer P |
80 mL |
180 mL |
| Buffer LS |
80 mL |
180 mL |
| Regeneration Buffer |
20 mL |
40 mL |
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